BS-010170-A03 | RTS-8 Plus eight position bioreactor for the non-invasive monitoring of cell growth in real-time by measurement of OD, pH and pO2








Key Features

  • Patented Reverse-Spin® technology non-invasively optimises mixing and oxygenation for aerobic cell cultivation
  • Measures OD, pH and pO2
  • Eight individually-controlled bioreactors for rapid bioprocess optimisation
  • Laser-based NIR optical system accurately determines OD levels to monitor cell growth kinetics
  • Utilises 50 mL Falcon tubes with membrane filters to provide a working media volume of 3 mL - 50 mL
  • Temperature control range from 15°C below ambient to +60°C
  • Active cooling for rapid temperature control
  • Real-time cell growth logging
  • Software included for storage, demonstration and analysis of data in real-time
  • Up to 12 RTS-8 Plus units can be simultaneously controlled by a PC through the software

Description

The RTS-8 Plus is an eight position bioreactor that utilises patented Reverse-Spin® technology to non-invasively optimise mixing and oxygenation for aerobic cell cultivation. With this novel technology a cell suspension in a single use 50 mL Falcon tube with a membrane filter is rotated around its axis in alternating directions to achieve a mass transfer coefficient (kLa (h-1)) of up to 450. This level of mass transfer is ideal for efficient aerobic cell culture.

The RTS-8 Plus features a laser-based optical system that emits NIR light at 850 nm. Highly accurate measurements of OD can be taken from 1 - 60 times per hour to provide a profile of cell growth in real-time. The temperature control range for the RTS-8 Plus is 15°C below ambient to +60°C.

Key applications for the RTS-8 Plus include:

  • Fermentation real time growth kinetics
  • Clone candidate screening
  • Protein expression
  • Temperature stress and fluctuation experiments
  • Media screening and optimisation
  • Growth characterisation
  • Inhibition and toxicity tests
  • Strain quality control
  • Initial bioprocess optimisation studies

Specifications

Light source
Laser
Measurement wavelength (λ)
850 ±15 nm
Measurement range
0 - 100 OD600
E. coli factory calibration measurement range
0 - 50 OD600
S. cerevisiae factory calibration measurement
range
0 - 75 OD600
Achievable user calibration measurement
error (range 0.1 - 6 OD600)
± 0.3
Achievable user calibration measurement
error (range 6 - 50 OD600)
≤ 5%
Achievable user calibration measurement
error (range 50 - 75 OD600)
≤ 10%
Measurement periodicity per hour
1 - 60
Temperature setting range
+15°C to +60°C
Temperature control range
15°C below ambient to +60°C
Temperature stability
±0.3°C
Sample temperature accuracy (20°C - 37°C)
±1°C
Tube sockets
8
Sample working volume range
3 mL - 50 mL
Speed control range
150 rpm - 2700 rpm
Reverse spin time setting range
150 rpm - 250 rpm
0 s
Reverse spin time setting range
250 rpm - 300 rpm
2 s - 60 s
Reverse spin time setting range
300 rpm - 2700 rpm
0 s - 60 s
Overall dimensions (W x D x H)
350 mm x 690 mm x 300 mm
Weight
20 Kg
Power supply
230 VAC: 50 Hz
Power consumption
3.15 A / 500 W


Sensor Specifications

O2 Sensor Specifications

Range
0 - 100%
Accuracy
±0.05% O2 at 0.2%
±0.4% O2 at 20.9%
Drift
<0.03% O2 within 30 days
Temperature range
Up to 40°C
Response time (t90)
<6 s
Storage stability
18 months


pH Sensor Specifications

Range
4.0 - 8.5 pH
Accuracy
±0.10 pH at pH 7
Drift
<0.005 pH per day
Operating temperature range
up to 40°C
Response time (t90)
<120 s
Storage stability
18 months


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About Us

Advance Scientific Products Pty Ltd is based in Queensland and is focused on delivering high-quality products and service to our customers in the life, chemical and analytical sciences across Australia.

The company was established in 2013 by experienced instrumentation specialists to provide full distribution and customer support capability to manufacturers seeking to expand their activities in Australia without having to create their own sales and marketing infrastructure.

Contact

PO Box 746
Moffat Beach, QLD, 4551, Australia