LEIA-X4 | Fluorescent quantitative detection system for real-time qPCR

Key Features

• Superb temperature control and accuracy
• Maximum heating rate of 5.0℃/s.
• Long-life LED light source with highly stable emission wavelength
• Multi-pixel photon counter (MPPC) detection
• Dynamic range of 1 - 10¹⁰ copies
• 4-channel fluorescence detection with no cross interference between channels
• No edge effect, no optical path correction, top excitation/detection, non-contact measurement
• Block temperature range of 4ºC - 99ºC
• Intuitive acquisition and analysis with flexible program setting, comprehensive analysis and reporting functions, and parameter storage capability.

Product Description

The LEIA-X4 fluorescent quantitative detection system for real-time qPCR utilises advanced Peltier systems to control sample temperature and optimise PCR amplification. The instrument features four fluorescent channels and LED excitation from 455nm-680nm to activate an extensive range of fluorescent dyes for quantitative detection. Emission signals are detected by a multi-pixel photon counter (MPPC) system in the range of 510nm - 730nm.

The LEIA-X4 utilises X/Y motor control to move the 96 wells of the sample plate to allow non-contact excitation/detection using top scanning of each well.

Image Acquisition and Analysis Software

The LEIA-X4 is controlled by intuitive and powerful software which also provides comprehensive data analysis capability. Analytical functions include absolute quantification, melting curve, relative quantification (AACT), and genotyping experiments. Guided flow operation, and the capability to open previous experimental records, allows for easy analysis, viewing of recent experiments, and the creation of new experiments.

More Information

Please click here to download a brochure describing the LEIA-X4 fluorescent quantitative detection system for real-time qPCR.

Specifications

Model number	LEIA-X4
Sample Capacity	96 x 0.1mL PCR plate, 12 x 8-strip tubes, 96 x 0.1mL single tubes
Sample volume	10 - 50µL
Dynamic range (copies)	1 - 1010
Fluorescent channels	4
Emission light	LED
Detector	Multi-pixel photon counter (MPPC)
Detection path | Suitable probe/dye	F1 | FAM/SYBR GREEN F2 | VIC/JOE/ HEX/TET F3 | ROX/TEXAS-RED F4 | Cy5
Excitation wavelength	455nm - 680nm
Detection wavelength	510nm - 730nm
Fluorescence detection repeatability	CV ≤2%
Fluorescence detection accuracy	CV ≤3%
Fluorescence detection linearity	r ≥ 0.995
Block temperature range	4 - 99℃ | 0.1℃ resolution
Maximum ramp rate	5.0℃/s
Temperature accuracy	± 0.3℃
Temperature uniformity	≤± 0.3℃
Temperature control mode	Block mode
Gradient temperature range	1°C - 36°C
Hot-lid maximum temperature	100℃ | automatic hot-lid
Scanning mode	Full plate scanning
Programming	Max 100 segments for each program | max 99 cycles
Operation mode	Continuous
Scanning Time	8.5s
Analytical functions	Absolute quantitative automatic analysis | relative quantification |SNP analysis | melting curve analysis
Operation System	Microsoft Windows 10
Electrical requirements	220V | 50/60Hz;
Instrument dimensions (L x W x H)	375mm x 490mm x 365mm
Communication	USB Port
Weight	45 Kg

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